생쥐 두개골에서 동종골 이식 시 면역억제에 대한 cyclosporine A의 효과
The immunosuppression effect of cyclosporine A on the allogenic calvarial bone graft in mice
김방신, 박상묵, 김경락, 정연욱, 한만승, 국민석, 박홍주, 유선열, 오희균,
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김방신 ( Kim Bang-Sin ) - 전남대학교 치의학전문대학원 구강악안면외과학교실
박상묵 ( Park Sang-Mook ) - 전남대학교 치의학전문대학원 구강악안면외과학교실
김경락 ( Kim Kyung-Rak ) - 전남대학교 치의학전문대학원 구강악안면외과학교실
정연욱 ( Jeong Youn-Wook ) - 전남대학교 치의학전문대학원 구강악안면외과학교실
한만승 ( Han Man-Seung ) - 전남대학교 치의학전문대학원 구강악안면외과학교실
국민석 ( Kook Min-Suk ) - 전남대학교 치의학전문대학원 구강악안면외과학교실
박홍주 ( Park Hong-Ju ) - 전남대학교 치의학전문대학원 구강악안면외과학교실
유선열 ( Ryu Sun-Youl ) - 전남대학교 치의학전문대학원 구강악안면외과학교실
오희균 ( Oh Hee-Kyun ) - 전남대학교 치의학전문대학원 구강악안면외과학교실
KMID : 0355620100360050353
Abstract
Introduction: This study examined the effect of cyclosporine A (CsA) on the allogenic cranial bone graft in the mice.
Materials and Methods:Twenty eight 12-week-old male ICR mice weighing 40 g were used. The experimental group was injected subcutaneously with CsA (10 ㎎/㎏/day) diluted in Caster oil for 7 days prior to the graft until sacrifice. The control group was injected with the same solution without CsA. Two full-thickness bone defects with a diameter of 3 ㎜ were made with a trephine bur in the parietal bone lateral to the sagittal suture. A calvarial defect of a mouse was grafted with allogenic calvarial bone disc from another mouse. The experimental and control groups were injected with CsA and the solution without CsA in the same manner before surgery, respectively. The mice were sacrificed at 1 week, 2 weeks and 4 weeks after the bone graft, respectively.
Results: In the experimental group, fibrous connective tissues and small amounts of infla㎜atory cells were observed. At 2 weeks after the allograft in the experimental group, new bone formation in fibrous collagenous tissue and around the allogenic bone was noted. At 4 weeks after the allograft, new bone formation was active along and at the periphery of the mature allogenic bone. The proliferation of blood vessels increased in bone marrow. In the control group, fibrous tissues and infla㎜atory cells were observed around the allogenic bone and existing bone at 1 week. At 2 weeks after the allograft, the proliferation of blood vessels accompanied by infla㎜atory cells were scattered in the fibrous connective tissues. New bone formation around the allogenic and existing bone could be observed. At 4 weeks after the allograft, infla㎜atory cells were severely infiltrated around the allogenic bone. Osteoclasts were scattered along the allogenic bone and induced bone resorption.
Conclusion: These results suggest that the daily administration of CsA (10 ㎎/㎏/day) induces efficient immunosuppression without serious complications ,and this protocol might be useful for the experimental model of allogenic bone grafts.
키워드
Cyclosporine A;Allogenic bone graft;Allograft;Immunosuppression;Osteoclasts
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